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Gene mutation assay of acrolein in the CHO/HGPRT test system
Author(s) -
Parent Richard A.,
Caravello Halina E.,
Harbell John W.
Publication year - 1991
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550110204
Subject(s) - acrolein , chinese hamster ovary cell , toxicity , chemistry , hypoxanthine guanine phosphoribosyltransferase , in vitro , microbiology and biotechnology , ames test , mutation , in vivo , genotoxicity , biochemistry , biology , gene , genetics , mutant , salmonella , catalysis , receptor , organic chemistry , bacteria
The mutagenic potential of acrolein has been studied with a wide range of in vitro and in vivo genetic toxicity assays. The data often have been conflicting, especially with the Ames assay. This study was undertaken to assess the mutagenic potential of acrolein using the CHO/HGPRT assay, both with and without metabolic activation. This assay system was chosen because it provides eukaryotic DNA as the target and is capable of detecting a range of mutational events. Because of its considerable toxicity, acrolein was tested over a very narrow dose range of 0.2–2 nl ml −1 without exogenous activation and 0.5–8 nl ml −1 with rat S‐9 activation. Multiple assays were performed under both conditions. The results indicated that while acrolein was clearly very cytotoxic, it did not induce a significant mutagenic response in the presence or absence of metabolic activation.