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Changes in urinary proximal tubule parameters in neonatal rats exposed to cadmium chloride during pregnancy
Author(s) -
Saillenfait A. M.,
Payan J. P.,
Brondeau M. T.,
Zissu D.,
de Ceaurriz J.
Publication year - 1991
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550110105
Subject(s) - offspring , endocrinology , medicine , urinary system , cadmium chloride , excretion , gestation , metallothionein , kidney , alkaline phosphatase , chemistry , fetus , pregnancy , toxicity , saline , cadmium , biology , enzyme , biochemistry , genetics , organic chemistry
Pregnant Sprague‐Dawley rats were injected intraperitoneally with physiological saline solution (vehicle) or cadmium chloride (CdCI 2 ) at 2.0 or 2.5 mg kg −1 on days 8, 10, 12 and 14 of gestation. On postnatal day (PND) 3, 12 or 49, the offspring were examined for 8‐ or 24‐h urinary excretion of β 2 ‐microglobulin (β 2 ‐m), metallothionein (MT) and urinary activity of three proximal tubular enzymes: gammaglutamyl transferase (GGT), alkaline phosphatase (ALP) and N ‐acetyl‐β‐glucosaminidase (NAG). Treatment with CdCI 2 did not affect growth or survival of offspring. Significant decreases in the urinary excretion of GGT, ALP and NAG were observed on PND 3, at both doses. Exposure to 4 × 2.5 mg kg −1 resulted in functional deficit of the proximal tubule on PND 3, as evidenced by the significant increase in β 2 ‐m. Except for a slight but significant increase of β 2 ‐m in 49‐day‐old males, all the other urinary parameters returned to control values on PND 12. There was no effect on MT. Results from this study show that prenatal exposure to CdCI 2 can induce significant changes in the kidney biochemistry of rats in the early postnatal period.