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Decrease in the rat bronchial acetylcholinesterase activity after toluene diisocyanate inhalation
Author(s) -
Brondeau M. T.,
Ban M.,
Simon P.,
Bonnet P.,
de Ceaurriz J.
Publication year - 1990
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550100608
Subject(s) - toluene diisocyanate , acetylcholinesterase , aché , inhalation , in vivo , chemistry , inhalation exposure , endocrinology , medicine , anesthesia , enzyme , biochemistry , biology , microbiology and biotechnology , organic chemistry , polyurethane
Acetylcholinesterase (AChE) activity was measured in bronchial tissue homogenate and blood from rats subjected to single and repeated 4‐h daily inhalation exposure to toluene diisocyanate (TDI) or control atmospheres. A single 4‐h exposure to TDI in the concentration range of 0.7–4.3 ppm did not decrease AChE activity in bronchial tissue but a 4‐h exposure to 0.6 ppm TDI, or greater, for two consecutive days did reduce this activity (19% to 33% of the controls). Increasing the level of exposure to TDI for two consecutive days from 0.6 to 4.0 ppm and extending the length of exposure to 1.2 ppm TDI from 2 to 4, 9 or 14 days produced no further decrease in bronchial ACHE activity. Throughout these experiments, blood AChE activity remained unchanged. In rats exposed to 0.3 or 1 ppm TDI for 3 weeks, staining of the bronchial smooth muscle for AChE was reduced (36% of the controls) after exposure to 1 ppm TDI. These results support, for the first time, in vivo and localized anti‐AChE activty by TDI.