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Biochemical and histopathological changes following dermal exposure to paraphenylene diamine in guinea pigs
Author(s) -
Mathur A. K.,
Gupta B. N.,
Narang S.,
Singh S.,
Mathur N.,
Singh A.,
Shukla L. J.,
Shanker Ravi
Publication year - 1990
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550100512
Subject(s) - lipid peroxidation , histamine , dermis , diamine oxidase , glutathione , hyperkeratosis , erythema , chemistry , medicine , endocrinology , pathology , enzyme , biochemistry , immunology
The guinea pigs were dermally exposed to paraphenylene diamine (PPI) at a dose level of 0.1 ml day −1 of a 1.0% (w/v) solution of PPD for 1, 3, 5 and 7 days. The absorption of PPD and its effects on lipid peroxidation, glutathione, histamine and several enzymes were assessed in skin and serum. Histopathological changes in liver, kidney and skin were examined also. The findings of the study indicated that PPD exposure resulted in significantly increased levels of lipid peroxidation and histamine contents in skin. The activity of enzymes increased significantly in skin and serum. PPD exposure also showed degenerative changes in liver and hyperkeratosis together with infiltration of cells in the dermis. Biochemical defects and histopathological changes in skin and serum correlated with the duration of exposure.