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The role of iron in t ‐butyl hydroperoxide‐induced lipid peroxidation and hepatotoxicity in rats
Author(s) -
Younes Maged,
Wess Alexander
Publication year - 1990
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550100502
Subject(s) - lipid peroxidation , chemistry , pharmacology , toxicity , biochemistry , oxidative stress , medicine , organic chemistry
Treatment of rats with 100 mg kg −1 t ‐butyl hydroperoxide led to an enhanced ethane exhalation as a marker of in vivo lipid peroxidation, as well as a moderate hepatoxicity as evidenced by a rise in plasma activities of liver‐specific enzymes (glutamate‐pyruvate transaminase and sorbitol dehydrogenase) and an increase in hepatic calcium content. Furthermore, a depletion of hepatic glutathione by 17% was observed. Apart from the loss of glutathione, all these effects were antagonized by pretreatment of rats with the potent iron chelator deferrioxamine and potentiated by pretreatment with low concentrations of FeSO 4 having no pro‐oxidant activity per se ; this was also the case in rats under conditions of iron overload (experimental haemochromatosis). These data indicate a close relationship between t ‐butyl hydroperoxide‐induced lipid peroxidation and its hepatotoxicity, and point out the importance of iron in catalysing reinitiation (propagation) reactions of lipid peroxidation in vivo .