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Cardioteratogenic dose of ethanol in the chick embryo results in egg white concentrations comparable to human blood alcohol levels
Author(s) -
Bruyere Harold J.,
Kapil Ram P.
Publication year - 1990
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550100113
Subject(s) - ethanol , embryo , alcohol , incubation , chemistry , toxicity , andrology , blood alcohol , medicine , endocrinology , chromatography , biology , biochemistry , poison control , microbiology and biotechnology , environmental health , injury prevention
Three‐day‐old chick embryos were exposed to a dose of ethyl alcohol (0.32 ml of 50% ethanol) that we previously demonstrated produces cardiac malformations in 96.6% of the animals. Ethanol was administered into the air sac at 72–80 h of incubation. Samples of egg white were drawn at 2, 6 and 24 h after treatment and analyzed by capillary gas–liquid chromatography. Ethanol concentrations were significantly higher at 6 and 24 h after exposure than at 2 h ( P < 0.01), but there were no differences in mean concentrations between 6 and 24 h ( P > 0.2). Furthermore, concentrations (43–303 mg dl −1 ) were comparable to human blood alcohol levels during intoxication. These results suggest that the cardioteratogenic doses of ethanol administered to chick embryos in a previous study are not excessive in terms of potential human embryo exposure.