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The effect of 2,4‐dichlorophenol on growth and plasmidic β‐lactamase activity in Escherichia Coli
Author(s) -
Espigares M.,
Mariscal A.
Publication year - 1989
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550090610
Subject(s) - escherichia coli , toxicant , microbiology and biotechnology , strain (injury) , pbr322 , plasmid , toxicity , enterobacteriaceae , chemistry , biology , biochemistry , dna , gene , organic chemistry , anatomy
The toxic effects of 2,4‐dichlorophenol on two strains of Escherichia coli harbouring plasmids with β‐lactamase activity were studied. The toxicant had a significant effect on growth at concentrations of 12 μg ml −1 for the JA 221 (pBR322) strain, as well as at 24 μg ml −1 for the J5‐3 (RP4) strain. β‐Lactamase production was affected at concentrations of 96 μg ml −1 in both strains. However, β‐lactamase production does not seem to constitute a reliable toxicity test in view of its variability and relatively low sensitivity.