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3‐Hydroxypropylmercapturic acid: A biologic marker of exposure to allylic and related compounds
Author(s) -
Sanduja Radhika,
Ansari G. A. S.,
Boor Paul J.
Publication year - 1989
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550090406
Subject(s) - acrolein , urine , allylamine , chemistry , high performance liquid chromatography , excretion , chromatography , detection limit , glutathione , biochemistry , organic chemistry , enzyme , polyelectrolyte , catalysis , polymer
3‐Hydroxypropylmercapturic acid [3‐OHPrMCA, S ‐(3‐hydroxypropyl)‐ N ‐acetyl‐ ‐l‐ ‐cysteine] was quantitatively measured by high‐performance liquid chromatography (HPLC) in the urine of rats given allylamine‐HCl (5, 25, 50, 100 and 150 mg kg −1 ), acrolein (13 mg kg −1 ), allylalcohol (64 mg kg −1 ), allylchloride (76 mg kg −1 ), allylbromide (120 mg kg −1 ), allylcyanide (115 mg) and cyclophosphamide (160 mg kg −1 ) by gavage in water. 3‐OHPrMCA was measured by HPLC in 24‐h urine collections; the lower detection limit was 1.25 μg or 5.6 nmol ml −1 . Various doses of allylamine resulted in 3‐OHPrMCA excretion at a fairly constant percentage of the dose, ca. 44–48% at 0–24 h and 3% at 24–48 h, indicating rapid metabolism through glutathione conjugation in the first 24h. Similarly, 3‐OHPrMCA was recovered in the urine of rats given acrolein (78.5%), allylalcohol (28.3%), allylchloride (21.5%), allylbromide (3.0%), allylcyanide (3.7%) and cyclophosphamide (2.6%). These data indicate that 3‐OHPrMCA can be used as a marker of exposure to allylic and other compounds that lead to the metabolic formation of acrolein.