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Tris (2,3‐dibromopropyl) phosphate nephrotoxicity in the rat: Histological and biochemical changes in renal components by 13 C‐NMR spectra
Author(s) -
Fukuoka Masamichi,
Tanaka Akira,
Yamaha Tsutomu,
Naito Katushi,
Takada Koichi,
Kobayashi Kazuo,
Tobe Masuo
Publication year - 1988
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550080605
Subject(s) - tris , nephrotoxicity , kidney , sialic acid , endocrinology , chemistry , medicine , lactate dehydrogenase , biochemistry , enzyme , biology
Abstract Rats receiving a single oral dose of 286.8 μmol/kg tris(2,3‐dibromopropyl)phosphate (Tris‐BP) showed pyknosis of the renal tubular epithelial cells on Day 1, necrosis on Day 2, regeneration from Day 3 and large nuclei formation from Day 4. 13 C‐NMR spectra were applied to clarify changes of the renal low‐molecular‐weight components in the kidney injured by Tris‐BP, and sialic acid and inositol were found as the desired marker components. The lesions produced by Tris‐BP were characterized by changes in the renal components and enzyme activities. Increases in sialic acid content of the kidney were observed both on Day 1, suggesting destruction of the epithelial cell membrane, and on Day 5, suggesting regeneration accompanied by an increase of inositol content on Days 7 and 10. Renal activity of cytoplasmic enzyme, alanine aminopeptidase, was decreased on Days 1 and 5 and elevated on Days 7 and 10. Lactate dehydrogenase activity showed a tendency to decrease in the kidneys on Day 1 and to increase significantly on Days 2, 5, 6 and 7.