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A simplified protocol for the mouse bone marrow micronucleus assay
Author(s) -
Garriott Michael L.,
Piper Charles E.,
Kokkino Andrew J.
Publication year - 1988
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550080212
Subject(s) - micronucleus test , clastogen , bone marrow , protocol (science) , micronucleus , in vivo , sampling (signal processing) , immunology , medicine , biology , toxicity , pathology , genetics , computer science , alternative medicine , filter (signal processing) , computer vision
Complex and expensive protocols involving multiple sampling times have been proposed and recommended for the in vivo mouse bone marrow micronucleus assay. The purpose of this study was to determine whether a simplified procedure employing two identical exposures and a single sampling time would be equally effective at detecting chemical clastogens. Furthermore, the utility of the protocol was investigated for both intraperitoneal and oral routes of administration. The results obtained from a group of chemicals spanning the known time range for maximum frequency of micronucleus induction prove the effectiveness of this simplified protocol.