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Studies of the in vitro effect of methylmercury chloride on rat brain neurotransmitter enzymes
Author(s) -
Kung MeiPing,
Kostyniak Paul,
Olson James,
Malone Maureen,
Roth Jerome A.
Publication year - 1987
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550070208
Subject(s) - choline acetyltransferase , enzyme , glutamate decarboxylase , chemistry , biochemistry , enolase , in vitro , glutamine , glutamine synthetase , pharmacology , biology , acetylcholine , amino acid , immunohistochemistry , immunology
The in vitro effect of methylmercury (MM) on the enzymatic activities of brain cell specific marker enzymes, choline acetyltransferase (CAT), glutamic acid decarboxylase (GAD), 2′, 3′‐cyclic nucleotide phosphohydrolase (CNP), glutamine synthetase (GS) and enolase was examined. The results demonstrate that at 100 μM MM, GS activity was not affected whereas a small decrease in the activity of both GAD (20%) and enolase (10%) was observed. CNP and CAT activity appeared to be more sensitive toward MM with 100 μM MM producing inhibition of 50% and 30%, respectively. The addition of sulfhydryl protecting reagents such as DTT or sodium thioglycolate can restore the enzyme activities to normal control levels despite prior exposure of the enzymes to MM.