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Uptake of cadmium in isolated kidney cells—influence of binding form and in vivo pretreatment
Author(s) -
Jin Taiyi,
Nordberg Gunnar F.,
Nordberg Monica
Publication year - 1986
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550060603
Subject(s) - metallothionein , cadmium , cysteine , albumin , chemistry , in vivo , kidney , toxicity , endocrinology , medicine , biochemistry , biology , enzyme , microbiology and biotechnology , organic chemistry
Uptake of cadmium as 109 CdCl 2 , 109 Cd‐cysteine, 109 Cd‐albumin and 109 Cd‐metallothionein was studied in isolated kidney cells from rat. Cd as 109 CdCl 2 and 109 Cd‐albumin was taken up at similar rates. The uptake of cadmium as 109 Cd‐cysteine was greater and that of 109 Cd‐metallothionein lower compared with that of the other substances. These observations were made on non‐pretreated cells. In cells taken from rats pretreated with CdCl 2 in vivo , the uptake of cadmium as 109 CdCl 2 , 109 Cd‐cysteine and 109 Cd‐albumin was lower than in cells from non‐pretreated rats. However, the uptake of 109 Cd‐metallothionein was considerably enhanced in pretreated cells. In pretreated kidney cells the decreased uptake of Cd (as Cd‐albumin) might be related to protection of the kidney against acute Cd toxicity and increased uptake of metallothionein‐Cd might contribute to the explanation of renal damage in long‐term Cd exposure.