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Measurement of the deposition and clearance of inhaled radiolabeled particles from rat lungs
Author(s) -
Newton Paul E.,
Pfledderer Chris
Publication year - 1986
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550060209
Subject(s) - deposition (geology) , nuclear medicine , lung , particle deposition , chemistry , geometric standard deviation , microsphere , radiochemistry , biomedical engineering , medicine , aerosol , paleontology , organic chemistry , chemical engineering , sediment , engineering , biology
Immediately after a 20 min nose only exposure to 51 Cr labeled polystyrene latex microspheres (1.4 MMAD, geometric standard deviation = 1.3, 2 μg m −3 ), 23% of the measured radioactivity was within the trachealungs of the exposed rats, 37% was within the gastrointestinal tract, 10% was within the defurred head, and 30% was on the fur. One hour after deposition these percentages had changed to 20, 64, 5 and 11% respectively. As a result of this non‐pulmonary particle load transiting the esophagus, external thoracic radioactivity measurements did not accurately reflect excised lung measurements until 30 h post‐deposition. Consequently, it was necessary to combine excised lung ratioactivity data (0–30 h post deposition) with serial external thoracic measurements (30–934 h post‐deposition) to measure the clearance of these microspheres. Use of an annulus shaped detector sufficiently increased sensitivity to allow measurements to be made through at least 934 h post‐deposition. The lung radioactivity retention curve was biexponential with half times of 34.3 ± 2.3 and 963 ± 107 h. Simultaneous exposures to 5% CO 2 increased deposition by 40% but failed to reduce deposition inhomogeneity and test variability.