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Urinary excretion products after the administration of 14 C‐acetaldehyde to rats
Author(s) -
Kallama Seija,
Hemminki Kari
Publication year - 1983
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550030608
Subject(s) - acetaldehyde , chemistry , urine , excretion , carboxylic acid , urinary system , chromatography , metabolism , ethanol , biochemistry , medicine
Rats were injected with 120 μCi of 14 C‐acetaldehyde containing carrier acetaldehyde, and the urinary excretion products were assayed by cation exchange chromatography, resolving five main peaks of radioactivity. Urine contained 6% of the dose of 14 C‐acetaldehyde administered. The main urinary product of acetaldehyde, fraction 5, was produced via acetate. Acetate was also identified in the urine. Two cysteine adducts, probably isomeric metabolites of 2‐methylthiazolidine‐4‐carboxylic acid, were detected. They constituted about 2% of the radioactivity in the urine collected 48 h after the administration of 14 C‐acetaldehyde. They appeared to retain a carboxylic group. When the reaction mixture of 14 C‐cysteine and acetaldehyde was injected into a rat, the half‐lives of the two tiazolidine derivatives was 10 h. Only 13.6% of the radioactivity was detected in the urine suggesting ready metabolism of 2‐methylthiazolidine‐4‐carboxylic acid.