Rapid high‐performance liquid chromatographic method for the estimation of toxicological levels of 25‐hydroxycholecalciferol (25‐OH D 3 ) in human serum | Zendy

Łukaszkiewicz Jacek | Zendy; Kozłowski Kazimierz | Zendy; Lorenc Roman | Zendy

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Rapid high‐performance liquid chromatographic method for the estimation of toxicological levels of 25‐hydroxycholecalciferol (25‐OH D 3 ) in human serum

Author(s) -

Łukaszkiewicz Jacek,

Kozłowski Kazimierz,

Lorenc Roman

Publication year - 1983

Publication title -

journal of applied toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.784

H-Index - 87

eISSN - 1099-1263

pISSN - 0260-437X

DOI - 10.1002/jat.2550030602

Subject(s) - chromatography , acetonitrile , high performance liquid chromatography , chemistry , extraction (chemistry) , solvent , hexane , vitamin d and neurology , medicine , endocrinology , organic chemistry

A reversed‐phase HPLC method for determination of 25‐hydroxycholecalciferol (25‐OH D 3 ) in serum samples is described. The method involves extraction of 25‐OH D 3 with hexane, followed by differential resolubilization in acetonitrile. The sample is then applied directly on the C 18 bonded phase column of the liquid chromatograph, and developed in a acetonitrile‐water solvent system. In the range 12.5–400 ng, a linear relationship was observed between detector response and the amount of 25‐OH D 3 placed on the column. Also, a very good correlation was observed between the amounts of 25‐OH D 3 added to serum samples, and the calculated amounts. The method can be used for screening for toxicological levels of 25‐OH D 3 in patients treated with high doses of vitamin D 3 .

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