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Comparison of toxic and transforming effects of ten environment‐related polycyclic hydrocarbons, including benzo [ a ] pyrene, on Fetal Hamster Lung Cell Cultures
Author(s) -
Emura M.,
RichterReichhelm H.B.,
Schneider P.,
Schoch C.,
Mohr U.
Publication year - 1982
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550020310
Subject(s) - fluoranthene , pyrene , chrysene , chemistry , benzopyrene , anthracene , benzo(a)pyrene , environmental chemistry , hamster , toxicity , stereochemistry , microbiology and biotechnology , toxicology , biology , organic chemistry
Standardized procedures were developed to compare quantitatively the toxic and transforming effects of ten environment‐related polycyclic aromatic hydrocarbons (PAH's) using early passage mixed cultures of fetal Syrian golden hamster lung cells. Evaluation of toxicity was attempted by applying a linear regression to the relative number of surviving colonies. Transformation data were fitted satisfactorily to the one‐hit model. Used as a monitor compound in each subexperiment, benzo[ a ]pyrene (BaP) showed dose‐related colony reduction (0.01–0.5 μg ml −1 ). Benzo[ b ]fluoranthene (BbF), indeno[1,2,3‐ cd ]pyrene (IcdP), benzo[ k ]fluoranthene (BkF) and benzo[ e ]pyrene (BeP) were generally colony enhancing at dose ranges up to 1.0 μg ml −1 . Fluoranthene (FLT), pyrene (PYR), chrysene (CHR), benzo[ ghi ]perylene ( Bghi PL) and benz[ a ]anthracene (BaA) were neither stimulatory nor inhibitory to colony formation. For transformation induction, BaP was over 14‐fold more effective than BbF, IcdP or BaA. The other PAH's showed no transformation.