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Homologous recombination induced by doxazosin mesylate and saw palmetto in the Drosophila wing‐spot test
Author(s) -
Gabriel Katiane Cella,
Dihl Rafael Rodrigues,
Lehmann Mauricio,
Reguly Maria Luiza,
Richter Marc François,
Andrade Heloisa Helena Rodrigues
Publication year - 2013
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1740
Subject(s) - mitotic crossover , doxazosin , genotoxicity , loss of heterozygosity , mutation , drosophila melanogaster , mesylate , genetics , microbiology and biotechnology , medicine , chemistry , endocrinology , pharmacology , biology , toxicity , gene , allele , organic chemistry , blood pressure
Benign prostatic hyperplasia (BPH) is the most common tumor in men over 40 years of age. Acute urinary retention (AUR) is regarded as the most serious hazard of untreated BPH. α ‐Blockers, such as doxazosin mesylate, and 5‐ α reductase inhibitors, such as finasteride, are frequently used because they decrease both AUR and the need for BPH‐related surgery. An extract of the fruit from American saw palmetto plant has also been used as an alternative ttreatment for BPH. The paucity of information available concerning the genotoxic action of these compounds led us to assess their activity as inducers of different types of DNA lesions using the somatic mutation and recombination test in Drosophila melanogaster . Finasteride did not induce gene mutation, chromosomal mutation or mitotic recombination, which means it was nongenotoxic in our experimental conditions. On the other hand, doxazosin mesylate and saw palmetto induced significant increases in spot frequencies in trans‐heterozygous flies. In order to establish the actual role played by mitotic recombination and by mutation in the genotoxicity observed, the balancer‐heterozygous flies were also analyzed, showing no increment in the total spot frequencies in relation to the negative control, for both drugs. Doxazosin mesylate and saw palmetto were classified as specific inducers of homologous recombination in Drosophila proliferative cells, an event linked to the loss of heterozygosity. Copyright © 2011 John Wiley & Sons, Ltd.