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Cardiotoxicity testing using pluripotent stem cell‐derived human cardiomyocytes and state‐of‐the‐art bioanalytics: a review
Author(s) -
Mandenius CarlFredrik,
Steel Daniella,
Noor Fozia,
Meyer Thomas,
Heinzle Elmar,
Asp Julia,
Arain Sarina,
Kraushaar Udo,
Bremer Susanne,
Class Reiner,
Sartipy Peter
Publication year - 2011
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1663
Subject(s) - cardiotoxicity , induced pluripotent stem cell , embryonic stem cell , stem cell , computational biology , computer science , medicine , biology , microbiology and biotechnology , toxicity , genetics , gene
In this article, recent progress in cardiotoxicity testing based on the use of immortalized cell lines or human embryonic stem cell (hESC) derived cardiomyocytes in combination with state‐of‐the‐art bioanalytical methods and sensors is reviewed. The focus is on hESC‐derived cells and their refinement into competent testing cells, but the access and utility of other relevant cell types are also discussed. Recent developments in sensor techniques and bioanalytical approaches for measuring critical cardiotoxicity parameters are highlighted, together with aspects of data evaluation and validation. Finally, recommendations for further research are given. Copyright © 2011 John Wiley & Sons, Ltd.