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Diphenyl diselenide potentiates nephrotoxicity induced by mercuric chloride in mice
Author(s) -
Brandão Ricardo,
Moresco Rafael N.,
Bellé Luziane P.,
Leite Marlon R.,
Freitas Mayara L.,
Bianchini Adalto,
Nogueira Cristina W.
Publication year - 2011
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1631
Subject(s) - chemistry , diphenyl diselenide , tbars , toxicity , nephrotoxicity , ascorbic acid , creatinine , urea , glutathione , kidney , hematocrit , medicine , endocrinology , selenium , biochemistry , enzyme , food science , organic chemistry
Following our long‐standing interest in the mechanisms involved in selenium toxicity, the aim of this work was to extend our previous studies to gain a better understanding of mercuric chloride (HgCl 2 ) + diphenyl diselenide (PhSe) 2 toxicity. Mice received one daily dose of HgCl 2 (4.6 mg kg −1 , subcutaneously) for three consecutive days. Thirty minutes after the last injection of HgCl 2 , mice received a single dose of (PhSe) 2 (31.2 mg kg −1 , subcutaneously). Five hours after (PhSe) 2 administration, mice were euthanized and δ ‐aminolevulinate dehydratase, catalase (CAT), glutathione S ‐transferase (GST) and Na + , K + ‐ATPase activities as well as thiobarbituric acid‐reactive substances (TBARS), ascorbic acid and mercury levels were determined in kidney and liver. Parameters in plasma (urea, creatinine, protein and erythropoietin), whole blood (hematocrit and hemoglobin) and urine (protein) were also investigated. HgCl 2 + (PhSe) 2 exposure caused a decrease in renal GST and Na + , K + ‐ATPase activities and an increase in renal ascorbic acid and TBARS concentrations when compared with the HgCl 2 group. (PhSe) 2 potentiated the increase in plasma urea caused by HgCl 2 . HgCl 2 + (PhSe) 2 exposure caused a reduction in plasma protein levels and an increase in hemoglobin and hematocrit contents when compared with the HgCl 2 group. There was a significant reduction in hepatic CAT activity and an increase in TBARS levels in mice exposed to HgCl 2 + (PhSe) 2 when compared with the HgCl 2 group. The results demonstrated that (PhSe) 2 did not modify mercury levels in mice. In conclusion, (PhSe) 2 potentiated damage caused by HgCl 2 affecting mainly the renal tissue. Copyright © 2011 John Wiley & Sons, Ltd.