Premium
Antigenotoxicity of artepillin C in vivo evaluated by the micronucleus and comet assays
Author(s) -
Azevedo Bentes Monteiro Neto Moacir,
Souza Lima Ildercílio Mota,
Furtado Ricardo Andrade,
Bastos Jairo Kenupp,
Silva Filho Ademar Alves,
Tavares Denise Crispim
Publication year - 2011
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1614
Subject(s) - comet assay , micronucleus test , micronucleus , propolis , in vivo , dna damage , pharmacology , biology , chemistry , microbiology and biotechnology , traditional medicine , toxicity , medicine , dna , genetics , organic chemistry
Artepillin C (3,5‐diprenyl‐ p ‐coumaric acid), a major compound found in Brazilian green propolis and Baccharis dracunculifolia , shows anti‐inflammatory, antibacterial, antiviral, antioxidant and antitumoral activities, among others. The aim of this study was to evaluate the genotoxic potential of artepillin C and its ability to prevent the chemically induced chromosome breakage or loss and the primary DNA damage using the micronucleus and comet assays in male Swiss mice, respectively. The animals were treated by gavage with different doses of artepillin C (0.4, 0.8 and 1.6 mg kg −1 b.w.). For the antigenotoxicity assays, the different doses of artepillin C were administered simultaneously to doxorubicin (DXR; micronucleus test; 15 mg kg −1 b.w.) and to methyl methanesulfonate (MMS; comet assay; 40 mg kg −1 b.w.). The results showed that artepillin C itself was not genotoxic in the mouse micronucleus and comet assays. In the animals treated with artepillin C and DXR, the number of micronucleated reticulocytes was significantly lower in comparison with the animals treated only with DXR. Regarding antigenotoxicity, artepillin C at the tested doses significantly reduced the extent of DNA damage in liver cells induced by MMS. Copyright © 2011 John Wiley & Sons, Ltd.