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In vitro effect of nickel on bovine spermatozoa motility and annexin V‐labeled membrane changes
Author(s) -
Lukac Norbert,
Bardos Laszlo,
Stawarz Robert,
Roychoudhury Shubhadeep,
Makarevich Alexander V.,
Chrenek Peter,
Danko Jan,
Massanyi Peter
Publication year - 2011
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1574
Subject(s) - motility , annexin , andrology , in vitro , chemistry , sperm motility , microbiology and biotechnology , biology , medicine , biochemistry
In this study the effect of in vitro culture of bovine spermatozoa with nickel (NiCl 2 ) on spermatozoa motility and membrane changes was analyzed. The spermatozoa motility significantly decreased after 120 min of culture at the concentration of 1000 μ m Ni ml −1 ( P < 0.05) and after 240 min of culture at the concentration of 500 and 1000 μ m Ni ml −1 ( P < 0.001) as compared with control. The progressive motility was the highest in the control group and in the groups with the lowest nickel concentrations (7.8 and 125 μ m Ni ml −1 ). The progressive spermatozoa motility was significantly altered even after 30 min of culture in the group with the highest nickel concentration (1000 μ m Ni ml −1 ). A significant decrease in progressive motility from the concentration of 250 μ m Ni ml −1 was detected after 240 min of culture. Concentrations from 125 μ m Ni ml −1 in various time periods of culture stimulated spermatozoa motility after 30 min ( P < 0.001), but later an inhibitory effect was noted. After 240 min of in vitro spermatozoa culture with 125 μ m Ni ml −1 a typical Annexin V fluorescence reaction was detected. Fluorescence was detected in mitochondrial segment of bovine spermatozoa. In spermatozoa exposed to higher nickel concentrations the Annexin V‐positive reaction was detected also on the spermatozoa head membrane. In the group with the highest concentration and the longest time of exposure (1000 μ m Ni ml −1 ; 240 min) the apoptotic Annexin‐positive regions were detected not only in the mitochondrial part, but also in the spermatozoa head (acrosomal and postacrosomal part), showing significant alteration of spermatozoa membrane integrity. Copyright © 2010 John Wiley & Sons, Ltd.