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Inhibitory effect of MIL glycoprotein on expression of pro‐inflammatory mediators in carbon tetrachloride‐induced mice liver damage
Author(s) -
Oh PhilSun,
Lee Jin,
Lim KyeTaek
Publication year - 2010
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1557
Subject(s) - tbars , carbon tetrachloride , liver injury , lactate dehydrogenase , chemistry , oxidative stress , pharmacology , thiobarbituric acid , tumor necrosis factor alpha , inflammation , lipid peroxidation , endocrinology , biochemistry , medicine , enzyme , organic chemistry
The aim of the present study was to evaluate immunomodulatory and hepatoprotective effects of glycoprotein isolated from Morus indica Linne (MIL glycoprotein) on carbon tetrachloride (CCl 4 )‐induced liver injury. In the present study, MIL glycoprotein (5 and 10 mg kg −1 body weight) was administered to ICR mice for 7 days prior to CCl 4 treatment. We evaluated the activities of alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and thiobarbituric acid‐reactive substances (TBARS), and expression of inflammation‐related mediators including cyclooxygenase (COX)‐2, tumor necrosis factor (TNF)‐ α , and interleukin (IL)‐1 β in CCl 4 ‐treated mice. Our results revealed that MIL glycoprotein reduced the activities of ALT, LDH and TBARS in serum from CCl 4 ‐treated mice. We also found that MIL glycoprotein reduced the activity of COX‐2 and expression of TNF‐ α and IL‐1 β in liver from CCl 4 ‐treated mice. Moreover, administration of MIL glycoprotein suppressed on stress‐activated protein kinase/c‐jun N‐terminal kinase phosphorylation and activator protein‐1 transcriptional activation in livers from CCl 4 ‐treated mice. The results from these experiments indicate that MIL glycoprotein effectively protects against liver injury, mainly through downregulation of oxidative stress and the inflammatory response. In conclusion, we suggest that the MIL glycoprotein might be one component of health supplements for prevention of liver diseases. Copyright © 2010 John Wiley & Sons, Ltd.