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Endogenous antioxidants and Nasal human epithelium response to air pollutants: genotoxic and inmmunocytochemical evaluation
Author(s) -
Fortoul T. I.,
RojasLemus M.,
AvilaCasado M. C.,
RodriguezLara V.,
Montaño L. F.,
MuñozComonfort A.,
LopezZepeda L. S.
Publication year - 2010
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1538
Subject(s) - genotoxicity , superoxide dismutase , oxidative stress , comet assay , catalase , dna damage , population , antioxidant , epithelium , pollutant , biology , toxicology , medicine , toxicity , biochemistry , environmental health , genetics , dna , ecology
Nasal epithelium is a source for identifying atmospheric pollution impact. Antioxidants play a relevant role in the protection of the cells from environmental injury, but scarce information is available about the interaction of endogenous antioxidants and genotoxic damage in nasal epithelium from urban populations highly exposed to traffic‐generated air pollutants. An immunocytochemical and genotoxic evaluation was implemented in nasal cell epithelium in a population chronically exposed to atmospheric pollution from autumn 2004 to autumn 2005. Superoxide dismutase (SOD) and Catalase (CAT) were evaluated in nasal scrapings by morphometry and genotoxicity by comet assay. An increase in DNA damage correlates with a decrease in SOD and CAT in nasal cells during autumn and the inverse result was observed during summer ( R = 0.88). Not only should exogenous antioxidant supplements be encouraged, but also a healthy diet to strengthen intracellular defenses against oxidative stress induced by exposure to air pollutants.