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Purified c‐phycoerythrin: safety studies in rats and protective role against permanganate‐mediated fibroblast‐DNA damage
Author(s) -
Soni Badrish,
Visavadiya Nishant P.,
Dalwadi Nirav,
Madamwar Datta,
Winder Chris,
Khalil Christian
Publication year - 2010
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1524
Subject(s) - mutagen , dna damage , phycoerythrin , comet assay , potassium permanganate , fibroblast , chemistry , toxicity , cytotoxicity , carcinogen , genotoxicity , microbiology and biotechnology , neutral red , in vitro , pharmacology , dna , biology , biochemistry , flow cytometry , organic chemistry
We have evaluated in vitro cytotoxicity of cyanobacterial phycoerythrin (C‐PE) on three human cell lines by cell proliferation and neutral red uptake assays. No toxic effects of C‐PE were observed to any of the cell lines tested. The protective role of purified C‐PE to potassium permanganate‐mediated human fibroblast‐DNA damage was assessed by comet assay at 0 (control), 10 and 20 µg C‐PE ml −1 doses in pre‐, simultaneous and post‐mutagen exposure conditions. Significant DNA damage was detected only in post‐mutagen exposure conditions. Our findings confirmed that the C‐PE is non‐toxic and provides protection against permanganate‐mediated DNA damage. The preliminary acute (2000 mg C‐PE kg −1 body weight, b.w.) and 90 day sub‐chronic (0, 5, 15 and 25 mg C‐PE kg −1 b.w./day) oral toxicity studies of purified C‐PE in male albino rats showed no mortality or treatment‐related major clinical signs, and all the doses of C‐PE were well tolerated. The no observed adverse effect level and no observed effect level were found to be 15 and 5 mg C‐PE kg −1 b.w./day respectively. Copyright © 2010 John Wiley & Sons, Ltd.