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Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays
Author(s) -
Furtado Ricardo Andrade,
de Araújo Felipe Rodrigues Rezende,
Resende Flávia Aparecida,
Cunha Wilson Roberto,
Tavares Denise Crispim
Publication year - 2010
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1491
Subject(s) - rosmarinic acid , micronucleus test , comet assay , dna damage , genotoxicity , antioxidant , pharmacology , chemistry , micronucleus , dna , toxicity , biochemistry , biology , organic chemistry
Rosmarinic acid (RA) is a naturally occurring phenolic compound, which contributes to the beneficial and health‐promoting effects of herbs, spices and medicinal plants. RA has shown several biological activities, such as hepatoprotective, anti‐inflammatory, antiangiogenic, antitumor, antidepressant, antineurodegenerative, HIV‐1 inhibitory and antioxidant effects. The aim of this study was to investigate the ability of RA to prevent chemically induced chromosome breakage or loss and primary DNA damage using the micronucleus and comet assays with V79 cells, respectively. The chemotherapeutic agent doxorubicin (DXR; 0.5 µg ml −1 ) was used as the DNA‐damaging agent. The cultures were treated with different concentrations of RA (0.28, 0.56 and 1.12 m m ) alone or in combination with DXR. The results showed that RA exerted no genotoxic effect, but significantly reduced the frequency of micronuclei and the extent of DNA damage induced by DXR at the three concentrations tested. The antioxidant activity of RA might be involved in the reduction of DXR‐induced DNA damage observed in the present study. Copyright © 2009 John Wiley & Sons, Ltd.