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Gene expression profiles in liver of mouse after chronic exposure to drinking water
Author(s) -
Wu Bing,
Zhang Yan,
Zhao Dayong,
Zhang Xuxiang,
Kong Zhiming,
Cheng Shupei
Publication year - 2009
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1441
Subject(s) - gene , kegg , biology , carcinogenesis , gene expression , gene expression profiling , signal transduction , carcinogen , biological pathway , oxidative stress , regulation of gene expression , fold change , genetics , transcriptome , biochemistry
cDNA micorarray approach was applied to hepatic transcriptional profile analysis in male mouse ( Mus musculus , ICR) to assess the potential health effects of drinking water in Nanjing, China. Mice were treated with continuous exposure to drinking water for 90 days. Hepatic gene expression was analyzed with Affymetrix Mouse Genome 430A 2.0 arrays, and pathway analysis was carried out by Molecule Annotation System 2.0 and KEGG pathway database. A total of 836 genes were found to be significantly altered (1.5‐fold, P ≤ 0.05), including 294 up‐regulated genes and 542 down‐regulated genes. According to biological pathway analysis, drinking water exposure resulted in aberration of gene expression and biological pathways linked to xenobiotic metabolism, signal transduction, cell cycle and oxidative stress response. Further, deregulation of several genes associated with carcinogenesis or tumor progression including Ccnd1 , Egfr , Map2k3 , Mcm2 , Orc2l and Smad2 was observed. Although transcription changes in identified genes are unlikely to be used as a sole indicator of adverse health effects, the results of this study could enhance our understanding of early toxic effects of drinking water exposure and support future studies on drinking water safety. Copyright © 2009 John Wiley & Sons, Ltd.