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Pentachlorophenol decreases ATP levels in human natural killer cells
Author(s) -
Nnodu Ugochukwu,
Whalen Margaret M.
Publication year - 2008
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1367
Subject(s) - pentachlorophenol , lytic cycle , glutathione , immune system , chemistry , biochemistry , pharmacology , biology , immunology , enzyme , virus , environmental chemistry
Pentachlorophenol (PCP) is used as a wood preservative and is found in human blood and urine. PCP causes significant decreases in the tumor‐killing (lytic) function of human natural killer (NK) cells, a critical immune defense. The current study examined the association between decreased lytic function and decreased ATP levels, as well as the ability of antioxidants (vitamin E and reduced glutathione) to prevent PCP‐induced decreases in either ATP levels or lytic function. Exposure of NK cells to 10 µ m PCP decreased ATP levels by 15% at 24 h, and exposure to 5 µ m PCP decreased ATP levels by 32% at 48 h. No effects were seen with 0.5 µ m at 48 h or with 5 µ m at 24 h. However, 10 µ m PCP decreased lytic function by 69% at 24 h and 5 µ m decreased it by 90% at 48 h. Even 0.5 µ m PCP decreased lytic function by 46% at 48 h. None of these effects were prevented by pretreatment with 1 m m vitamin E or reduced glutathione. Copyright © 2008 John Wiley & Sons, Ltd.