Study on the  in vitro  effect of zearalenone and  α ‐zearalenol on boar sperm–zona pellucida interaction by hemizona assay application | Zendy

Tsakmakidis I. A. | Zendy; Lymberopoulos A. G. | Zendy; Vainas E. | Zendy; Boscos C. M. | Zendy; Kyriakis S. C. | Zendy; Alexopoulos C. | Zendy

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Study on the in vitro effect of zearalenone and α ‐zearalenol on boar sperm–zona pellucida interaction by hemizona assay application

Author(s) -

Tsakmakidis I. A.,

Lymberopoulos A. G.,

Vainas E.,

Boscos C. M.,

Kyriakis S. C.,

Alexopoulos C.

Publication year - 2007

Publication title -

journal of applied toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.784

H-Index - 87

eISSN - 1099-1263

pISSN - 0260-437X

DOI - 10.1002/jat.1239

Subject(s) - zona pellucida , zearalenone , semen , boar , sperm , andrology , acrosin , oocyte , in vitro , chemistry , biology , mycotoxin , acrosome , biochemistry , embryo , microbiology and biotechnology , medicine , food science

The mycotoxin zearalenone (zen) impairs fertility in farm animals. The aim of the present study was to investigate the effect of zearalenone and its major metabolite ( α ‐zearalenol) on boar semen binding capacity, under in vitro conditions. Extended boar semen was exposed to three different concentrations of zen and α ‐zen (40, 60 and 80 µg ml −1 of semen) for 1 h. Afterwards, the semen was washed and incubated with homologous oocyte hemizona for 4 h. A significant decrease ( P < 0.001) in the number of tightly attached spermatozoa on the hemizona was obtained at concentrations of 60 µg ml −1 and 80 µg ml −1 of zen and α ‐zen. In conclusion, zen and α ‐zen affected the sperm–zona interaction by reducing the ability of boar spermatozoa to bind to the zona pellucida. Copyright © 2007 John Wiley & Sons, Ltd.

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