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A battery of DNA effect biomarkers to evaluate environmental exposure of Flemish adolescents
Author(s) -
Koppen G.,
Verheyen G.,
Maes A.,
Gorp U. Van,
Schoeters G.,
Hond E. Den,
Staessen J.,
Nawrot T.,
Roels H. A.,
Vlietinck R.,
Verschaeve L.
Publication year - 2007
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1174
Subject(s) - comet assay , genotoxicity , chemistry , micronucleus , dna damage , micronucleus test , benzo(a)pyrene , pyrene , urine , toxicology , environmental chemistry , microbiology and biotechnology , dna , biochemistry , biology , toxicity , organic chemistry
Abstract The present paper deals with the evaluation of a battery of genotoxicity biomarkers in healthy Flemish adolescents and their relation with common pollutants occurring in their life environment. DNA damage as reflected by the comet assay appeared to be most sensitive to ozone (partial r 2 = 0.102, p < 0.00001), and to a lesser extent to ortho‐cresol (partial r 2 = 0.055; p = 0.001) and 1‐hydroxy‐pyrene (1‐OH‐pyrene, partial r 2 = 0.031; p = 0.013). 8‐hydroxy‐deoxyguanosine (8‐OHdG) was only related to ortho‐cresol ( r 2 = 0.069; p < 0.007). Interestingly, the comet assay results and urinary 8‐OHdG concentrations were positively correlated with a Pearson r = 0.21 ( p = 0.003, N = 200). Logistic regression models revealed significant relations between chromatid breaks and 1‐OH‐pyrene (relative risk (RR): 1.58; p = 0.008), and t,t‐muconic acid (RR: 1.71; p = 0.014). There was no correlation between micronucleus formation or occurrence of chromosomal or chromatid breaks on the one hand and comet or 8‐OHdG results on the other hand. Thus, in this study the comet assay on whole blood samples and urine 8‐OHdG measurements especially appeared sensitive biomarkers for assessing the genetic effects of environmental pollutants to which adolescents may be exposed. Copyright © 2007 John Wiley & Sons, Ltd.