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Genotoxic and aneugenic properties of an imidazole derivative
Author(s) -
Carballo M. A.,
Hick A. S.,
Soloneski S.,
Larramendy M. L.,
Mudry M. D.
Publication year - 2006
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1132
Subject(s) - chinese hamster ovary cell , mitotic index , genotoxicity , sister chromatid exchange , spindle apparatus , chemistry , lymphocyte , carcinogen , multipolar spindles , mitosis , toxicology , sister chromatids , microbiology and biotechnology , toxicity , biology , pharmacology , genetics , biochemistry , cell culture , in vitro , cell , cell division , chromosome , organic chemistry , gene
To contribute to a more accurate characterization of the mutagenic and aneugenic effects of thiabendazole (TBZ), a widely used antiparasitic and food preservative drug, the induction of sister chromatid exchanges (SCEs) and mitotic spindle anomalies as cytogenetic end‐points were investigated. Studies were carried out in Chinese hamster ovary (CHO) cells and human peripheral blood lymphocytes. A significant dose‐dependent increase in SCE frequency was observed in CHO cells with S9‐Mix ( P < 0.01) in the 50–100 µg ml −1 dose‐range, while in the absence of S9‐Mix, an enhancement of the SCE frequency was exhibited at the highest dose ( P < 0.01). In CHO‐K1 cells a significant increase in mitotic spindle anomalies ( P < 0.01) was observed with the highest concentration assayed reflecting the specific effect of TBZ formulation at the microtubule level. Cell proliferation kinetics (CPK) were not modified by the addition of this pharmaceutical product. In human lymphocyte cultures, exposure to 100 µg ml −1 TBZ formulation resulted in a significant decrease of the mitotic index (MI) ( P < 0.003) and changes in the replication index (RI) ( P < 0.05). Copyright © 2006 John Wiley & Sons, Ltd.