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Responses of fish chromatophore‐based cytosensor to a broad range of biological agents
Author(s) -
Dierksen Karen P.,
Mojovic Ljiljana,
Caldwell Bruce A.,
Preston R. Ryan,
Upson Rosalyn,
Lawrence Jeannine,
McFadden Philip N.,
Trempy Janine E.
Publication year - 2004
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.1030
Subject(s) - streptolysin , biology , microbiology and biotechnology , chromatophore , toxin , vibrio , effector , vibrio parahaemolyticus , biochemistry , bacteria , bacterial protein , fishery , genetics , gene
A cytosensor based on living chromatophores from Betta splendens Siamese ghting sh was used to test several classes of biologically active agents. Tested agents include neurotransmitters, adenyl cyclase activators, cytoskeleton effectors, cell membrane effectors and protein synthesis inhibitors. Characteristic cell responses were analyzed, and potential cytosensor applications were considered. Streptococcus pyogenes toxins streptolysin S and streptolysin O, Clostridium tetani tetanolysin, Staphylococcus aureus alpha‐toxin and Vibrio parahemolyticus hemolysin, all bacterial toxins that act on cell membranes, elicited a strong response from chromatophores. A comparison of puried toxin to actual bacterial culture from Vibrio parahemolyticus demonstrated a nearly identical chromatophore cell response pattern. This suggests that the cytosensor response is reective of bacterial toxin production. Copyright © 2004 John Wiley & Sons, Ltd.