Open AccessExpression of NGF and its functional implication in the lung injury induced by cerebral ischemia
Author(s) -
Zhang LanChun,
Hu Yue,
Xia QingJie
Publication year - 2018
Publication title -
ibrain
Language(s) - English
Resource type - Journals
eISSN - 2769-2795
pISSN - 2313-1934
DOI - 10.1002/j.2769-2795.2018.tb00020.x
Subject(s) - lung , nerve growth factor , h&e stain , immunohistochemistry , medicine , pathology , ischemia , western blot , edema , pulmonary edema , biology , receptor , biochemistry , gene
Background The purpose of this study is to investigate the expression of nerve growth factor (NGF) in the lung injury induced by cerebral ischemia. Methods Adult healthy female Sprague‐Dawley rats were randomly divided into two groups: sham group and brain ischemia (BI) induced lung injury group. Lung injury was induced by BI and neurological severity score (NSS) was evaluated. Triphenyltetrazolium chloride (TTC) staining, brain edema, ratio of wet/dry of lung tissue, hematoxylin‐eosin (HE) staining, as well as pulmonary edema were measured, respectively. The expression of mRNA and protein of NGF in lung tissues was detected by qPCR and western blot at 14 days post injury respectively. The immunohistochemical method was used to localize the expression of NGF in the lung tissues. Results Lung injury from BI was successfully induced. The localization of NGF was detected mainly in the lung epithelial tissue following BI, also in vascular endothelial tissues. Compared with the sham group, the expression level of mRNA and protein for NGF was increased significantly after BI. Conclusion The increase of NGF in the lung tissue after BI showed that NGF has been involved in the repair of injured lung, and the underlying mechanism of BI induced lung injury.