Open AccessInterference chemically synthesized dsRNA decreased IL‐1β expression in PC12 cells and its functional implication
Author(s) -
Dan QiQin,
Xia QingJie,
Wang TingHua
Publication year - 2017
Publication title -
ibrain
Language(s) - English
Resource type - Journals
eISSN - 2769-2795
pISSN - 2313-1934
DOI - 10.1002/j.2769-2795.2017.tb00014.x
Subject(s) - rna interference , gene silencing , transfection , rna silencing , gene expression , in vitro , interference (communication) , biology , microbiology and biotechnology , gene , rna , genetics , computer science , telecommunications , channel (broadcasting)
Objective RNA interference (RNAi), a kind of gene silence, could regulate gene expression so as to be used as a useful technology for the treatment of disease. IL‐1β, a proinflammatory cytokine, plays a significant role in diverse diseases. This study aimed to explore the detailed protocol for the inhibition of IL‐ 1β, which is avaiable for its preclinic usage. Methods We used PC12 cells to explore the effective interference fragment of IL‐1β in vitro , and quantitative‐PCR (qPCR) was used to validate the expression of IL‐1β. Results The transfection efficiency of IL‐1β was more than 90% with the concentration of 50nM and 80 nM qPCR, it showed the most effective interference fragment for IL‐1β in PC12 cells was F3, which may be useful to pre‐clinic trial in future. Conclusion PC12 cells were employed to screen the effective interference fragments for IL‐1β and confined the most effective interference fragment as F3 which could be served as the regulating agent of IL‐1β in future pre‐clinic trial.