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New International Calibration Standard (ICE‐3) for HPLC Analysis of α‐ and β‐acids
Author(s) -
Biendl Martin
Publication year - 2010
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2010.tb00418.x
Subject(s) - calibration , chromatography , high performance liquid chromatography , chemistry , environmental science , mathematics , statistics
In 1996 the EBC and the ASBC released the first “International Calibration Extract” (ICE-1) for HPLC analysis of αand β-acids in hops and hop products. A joint EBC/ASBC Hop Standard Sub-Committee was established to monitor the stability of ICE-1. The results of a check carried out in spring 1997 convinced the Sub-Committee to plan the release of a new hop standard. To guarantee the best possible stability of the successor the Working Group of Hop Analysis (AHA) tested in extensive storage trials at –20°C and +40°C several extracts of varying composition each one filled in aluminum, tin, plastic or glass vials. On the basis of the results received it was decided to choose as best suited for ICE-2 the de-oiled supercritical CO2-extract “Hallertau Perle” filled in glass vials. This standard was then released by September 1st, 1998. Since that time, the stability of ICE-2 has been checked every 6 months by the Working Group of Hop Analysis (AHA) and this standard proved to be stable for more than 10 years until today.