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A Study of Saccharomyces cerevisiae Cell Wall Glucans
Author(s) -
Kwiatkowski Stefan,
Thielen Ursula,
Glenney Phyllis,
Moran Colm
Publication year - 2009
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2009.tb00361.x
Subject(s) - glucan , polysaccharide , saccharomyces cerevisiae , cell wall , yeast , biochemistry , monoclonal antibody , chemistry , starch , glycogen , biology , antibody , immunology
Much research has been carried out over the years examining cell wall glucans from Saccharomyces cerevisiae and this study further examines aspects of the binding of (1r̊4)‐α‐D‐glucan in the yeast cell wall, using a number of isolation techniques as well as monoclonal antibodies able to recognize a mixed (1r̊4)‐α‐D‐glucan/(1r̊6)‐β‐D‐glucan. Extraction of purified glucan, from S. cerevisiae cell wall, with 0.1N HCl, at 80°C for 6 h, released into the solution (1r̊4)‐α‐D‐glucan and (1r̊6)‐β‐D‐glucan as the major polysaccharides, along with an insoluble pellet highly enriched in (1r̊3)‐β‐D‐glucan. The released (1r̊4)‐α‐D‐glucan was composed of a high molecular size >100 kDa fraction (7.2% w/w) and a medium 5–50 kDa polysaccharide (10.2% w/w), with the (1r̊4)‐α‐D‐glucan covalently bound to the (1r̊6)‐β‐D‐glucan. The average molar ratio of the α:β glucan was 47: 53 in this mixed polysaccharide. The structure of this polysaccharide was different from the structure of plant starch or animal glycogen as monoclonal antibodies specific to yeast (1r̊4)‐α‐D‐glucan/(1r̊6)‐β‐D‐glucan did not recognize the plant starch or animal glycogen standards.