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The Yeast Vacuole ‐A Scanning Electron Microscopy Study During High Gravity Wort Fermentations
Author(s) -
Pratt Patricia L.,
Bryce James H.,
Stewart Graham G.
Publication year - 2007
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2007.tb00256.x
Subject(s) - high gravity , vacuole , yeast , fermentation , specific gravity , chemistry , ethanol , food science , scanning electron microscope , biochemistry , mineralogy , materials science , cytoplasm , composite material
The yeast vacuole has been shown to exhibit morphological responses to environmental conditions when exposed to worts of different gravity during fermentation. Marked effects of high gravity wort (20° Plato) on yeast morphology compared to more conventional wort gravity (12° Plato) were observed. High gravity worts caused vacuolar enlargement compared to conventional gravity wort. These results suggested that yeast cells experienced severe alterations with the vacuolar tonoplast when exposed to high osmotic pressure and elevated levels of ethanol.