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PCR‐Based Differentiation and Homology of Brewing and Type Strains of the Genus Saccharomyces
Author(s) -
Barszczewski Wojciech,
Robak Małgorzata
Publication year - 2006
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2006.tb00246.x
Subject(s) - haeiii , rapd , biology , restriction fragment length polymorphism , genetics , restriction enzyme , saccharomyces cerevisiae , homology (biology) , saccharomyces , primer (cosmetics) , yeast , gene , polymerase chain reaction , genetic diversity , population , chemistry , demography , organic chemistry , sociology
Restriction fragment length polymorphism (RFLP) patterns of PCR‐amplified ribosomal RNA gene fragments (rDNA) and randomly amplified polymorphic DNA (RAPD) were applied for the analysis of 15 brewing and 6 related yeast strains of the genus Saccharomyces. One five‐base ( Scr FI) and two four‐base cutting ( Hae III, Msp I) restriction enzymes were used. The primers 21 and M13 core sequence were selected for RAPD analysis. PCR‐RFLP rDNA analysis with Hae III, Scr FI and Msp I differentiated the strains tested into four, five and four types of patterns, respectively and the analyses of the profiles showed 100% homology, between the yeast strains. One strain was an exception. Homological groups were observed for strains used in breweries globally, from a local production strain and from the isolates identified as S. cerevisiae. Using RAPD analysis, and according to discrete differences in the profiles, it was possible to divide twenty one strains into 15 and 20 groups with primer 21 and M13 respectively. RFLP‐PCR rDNA analysis was used to show similarities in closely related brewing strains, while RAPD analysis was used for differentiation of strains.