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The Potential of Confocal Imaging for Measuring Physiological Changes in Brewer's Yeast
Author(s) -
Schlee Cornelia,
Miedl Michaela,
Leiper Kenneth A.,
Stewart Graham G.
Publication year - 2006
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2006.tb00243.x
Subject(s) - yeast , brewing , trehalose , confocal , saccharomyces cerevisiae , fermentation , biochemistry , biology , confocal microscopy , saccharomyces , fluorescence microscope , confocal laser scanning microscopy , glycogen , microbiology and biotechnology , fluorescence , chemistry , biophysics , physics , geometry , mathematics , quantum mechanics
ABSTRACT This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation.