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Comparison of Esterase Patterns of Three Yeast Strains As Obtained with Different Synthetic Substrates
Author(s) -
Lomolino Giovanna,
Lante Anna,
Rizzi Corrado,
Spettoli Paolo,
Curioni Andrea
Publication year - 2005
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2005.tb00672.x
Subject(s) - esterase , yeast , chromogenic , carboxylesterase , biochemistry , yeast in winemaking , chemistry , substrate (aquarium) , biology , saccharomyces cerevisiae , chromatography , enzyme , ecology
The visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity was performed by using different chromogenic and fluorescent substrates. The zymograms revealed the presence of several isoforms in yeast extracts, differing in sub‐cellular location, substrate specificity and electrophoretic mobility, indicating a high level of polymorphism.