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Malting Barley Grain Non‐specific Lipid‐Transfer Protein (ns‐LTP): Importance for Grain Protection
Author(s) -
Gorjanović Stanislava,
Spillner Edzard,
Beljanski Miloš V.,
Gorjanović Radmila,
Pavlović Mirjana,
GojgićCvijanović Gordana
Publication year - 2005
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2005.tb00654.x
Subject(s) - plant lipid transfer proteins , fusarium , hordeum vulgare , fusarium solani , candida albicans , incubation , long term potentiation , biology , food science , saccharomyces cerevisiae , chemistry , biochemistry , botany , poaceae , yeast , gene , receptor
A basic protein (pI < 9) was isolated to homogeneity from a domestic cultivar of malting barley grain ( Hordeum vulgare ). In its unreduced form it exists as a dimer of a 9 kDa protomer with four disulphide bridges. These characteristics together with protein sequence data revealed that the isolated protein belongs to the class of ns‐LTP. The antifungal potency of malting barley grain ns‐LTP was examined on Saccharomyces cerevisiae, Candida albicans and the plant pathogen Fusarium solani growth in vitro . It was found that ns‐LTP inhibits Saccharomyces and Fusarium growth; the concentration required for 50% inhibition after 24 h of incubation (IC 50 ) was 100 and 80 μg/mL, respectively. On the basis of these results, the importance of ns‐LTP for barley grain protection from fungal diseases has been discussed.