Premium
Distinctly Different Characteristics of Flocculation in Yeast 1
Author(s) -
Nishihara H.,
Miyake K.,
Kageyama Y.
Publication year - 2002
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2002.tb00539.x
Subject(s) - flocculation , yeast , chemistry , biochemistry , microbiology and biotechnology , periodate , biology , organic chemistry
Flocculation of cells of S. cerevisiae NCYC 1109 and S. cerevisiae NCYC 234 was studied. Both cells flocculated 48 h after inoculation although the cells grown for only 20 h were non‐flocculent. EDTA inhibited the flocculation of cells of S. cerevisiae NCYC 1109 and Ca 2+ enhanced it. Protein‐denaturants, D‐mannose and D‐glucose depressed the flocculation of cells of NCYC 1109. Treatment with proteolytic enzymes, photo‐irradiation in the presence of methylene blue or periodate oxidation caused a loss of the floc‐forming ability of the cells of NCYC 1109. On the contrary, the flocculation of cells of NCYC 234 was not affected by EDTA and Ca 2+ . Protein‐denaturants and monosaccharides did not inhibit the flocculent ability of cells of NCYC 234. Neither proteolytic enzyme treatments, photo‐irradiation in the presence of methylene blue nor periodate oxidation, deprived the cells of NCYC 234 of the floc‐forming ability. Cycloheximide repressed induction of the floc‐forming ability of growing cells of NCYC 1109 significantly but not of growing cells of NCYC 234. These results suggest that the mechanisms of flocculation of NCYC 1109 and NCYC 234 are quite different.