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Enzymic Digestion of Walls Purified from the Starchy Endosperm of Barley
Author(s) -
Kanauchi M.,
Bamforth C.W.
Publication year - 2002
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.2002.tb00127.x
Subject(s) - endosperm , ferulic acid , glucan , digestion (alchemy) , cell wall , limiting , chemistry , biochemistry , food science , hydrolysis , acetic acid , chromatography , mechanical engineering , engineering
Cell walls have been purified from the starchy endosperm of barley. Xylanases can effect the release of β‐glucan from these walls, but β‐glucanases do not release pentosan. Feruloyl esterase and xyloacetylesterase, with commensurate release of ferulic acid and acetic acid respectively, can effect some solubilisation of glucan. Digestion of the pentosan layer and of the feruloyl and acetyl esterification does not appear to be a prerequisite for glucan degradation. However, no more than 15% of the total β‐glucan in the wall could be extracted, suggesting that some factor other than one identified in this study is limiting the solubility of this polymer.