Premium
The Application of Near Infrared Spectroscopy to Evaluate Malting Quality
Author(s) -
Ratcliffe M.,
Panozzo J.F.
Publication year - 1999
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1999.tb00010.x
Subject(s) - near infrared spectroscopy , calibration , cultivar , standard error , peristaltic pump , standard deviation , sampling (signal processing) , near infrared reflectance spectroscopy , analytical chemistry (journal) , mathematics , filter (signal processing) , chemistry , computer science , statistics , agronomy , chromatography , biology , engineering , mechanical engineering , neuroscience , computer vision
Near infrared (NIR) spectroscopy is a rapid technique which is used within plant breeding programs for the analysis of many grain traits. This study investigated the application of NIR analysis of wort samples to select malting quality cultivars in a barley breeding program. An automatic sampling and data capture system was developed which consisted of a Perten filter NIR instrument with a flow through cell module operating in transflectance mode interfaced with a computer, peristaltic pump and sample changer. Calibrations for hot water extract, free alpha‐amino nitrogen and soluble protein were developed using multiple linear regression analysis based on four wavelength terms for each trait. The correlation coefficients for both calibration and prediction data sets were highly significant (P<0.01) and the standard error of prediction was similar to that obtained by standard methods. Cultivars with known malting quality were included in the experiments and their ranking by NIR was consistent with the standard methods. The reported calibrations have been used for over four years to screen early generation breeding lines for malting quality.