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A STUDY OF VIABILITY AND TREHALOSE METABOLISM IN SACCHAROMYCES CEREVISIAE VACUOLAR DEFICIENT MUTANT SKD2
Author(s) -
Gent Daniel P.,
Morimura Shigeru,
Ujhe Ikuyo,
Kida Kenji
Publication year - 1997
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1997.tb00968.x
Subject(s) - trehalase , trehalose , vacuole , biochemistry , saccharomyces cerevisiae , sugar phosphates , cytosol , enzyme , mutant , yeast , sugar , intracellular , biology , acid phosphatase , chemistry , gene , cytoplasm
The vacuolar deficient mutant SKD2 and a closely related vacuole complete strain SKD1ρ − were studied to identify the cause of low trehalose accumulation in SKD2. The present work indicates that low intracellular trehalose levels in SKD2 result from reduced activity of enzymes of the trehalose‐6‐phosphate synthase/phosphatase complex rather than higher activity of trehalase in the cytosol caused by supposed ineffective compartmentalisation of vacuolar acid trehalase. In vitro studies using crude enzyme extracts from SKD2 and SKD1ρ − also suggested that loss of viability of SKD2 might result from a fall in free phosphate levels as sugar phosphates accumulated .