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RAPID YEAST TREHALOSE MEASUREMENT USING NEAR INFRARED REFLECTANCE SPECTROMETRY
Author(s) -
MOONSAMY N.,
MOCHABA F.,
MAJARA M.,
O'CONNORCOX E. S. C.,
AXCELL B. C.
Publication year - 1995
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1995.tb00863.x
Subject(s) - slurry , trehalose , yeast , reflectivity , calibration , near infrared spectroscopy , chromatography , mass spectrometry , reproducibility , chemistry , glycogen , analytical chemistry (journal) , materials science , biochemistry , biology , optics , mathematics , physics , statistics , neuroscience , composite material
The rapid measurement of yeast trehalose by Near Infra‐Red Reflectance (NIR) spectrometry is demonstrated for both dried and slurry samples. The reproducibility of the method was acceptable considering the errors in both the enzymatic calibration and determination of cell dry weight methods. The results were better in the case of slurry samples presumably because the error in the drying method was removed. Direct slurry measurement is also more rapid and more desirable for future inline applications. Further improvements in the measurement of yeast glycogen by NIR as previously published are also reported. NIR reflectance would appear to be a promising technique for the rapid in‐line measurement of yeast intracellular components within a brewery.