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IDENTIFICATION OF PROANTHOCYANIDINS IN BEER AND THEIR DIRECT MEASUREMENT WITH A DUAL ELECTRODE ELECTROCHEMICAL DETECTOR
Author(s) -
McMurrough I.,
Baert T.
Publication year - 1994
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1994.tb00839.x
Subject(s) - chemistry , proanthocyanidin , polyphenol , chromatography , amperometry , electrochemistry , electrode , organic chemistry , antioxidant
A sensitive and reliable method was developed for the measurement of simple flavanols in beer by direct‐injection High Performance Liquid Chromatography using dual‐electrode electrochemical detection. When operated in the amperometric mode the detector was extremely sensitive for readily‐oxidized substances such as polyphenols, so that sample preparation was minimal. The dimeric flavanols in a commercial beer were positively identified as procyanidin B3 and prodelphinidin B3. Tentative identifications for three trimeric proanthocyanidins were made also. Collectively, monomeric, dimeric and trimeric flavanols accounted for only about 4% of the colorimetrically determined total polyphenol content, and about 35% of the total flavanol content of a non‐haze stabilized beer. The improved chromatographic method was applied to measuring the decreases in simple flavanols caused by treatment of beer with polyvinylpolypyrrolidone.