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ALPHA‐ACID—1,2‐DIAMINOBENZENE COMPLEX: A CONVENIENT STANDARD FOR ALPHA‐ACID ANALYSIS
Author(s) -
Green Colin P.,
Osborne Peter
Publication year - 1993
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1993.tb01174.x
Subject(s) - alpha (finance) , chemistry , high performance liquid chromatography , chromatography , ion exchange resin , organic chemistry , mathematics , construct validity , statistics , psychometrics
A convenient preparation of the complex of alpha‐acid and 1,2‐diaminobenzene (o‐phenylene diamine) is given. Use of the complex as an external standard for HPLC analysis of hops is described. Pure alpha‐acid can be recovered from the complex by treatment with ion‐exchange resin. The complex is stable in the absence of light and can easily be made in sufficient quantity and purity for distribution as a primary alpha‐acid standard.