Premium
RESTRICTION MAPPING OF THE POF I GENE
Author(s) -
Hwang Angela
Publication year - 1992
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1992.tb01131.x
Subject(s) - saccharomyces cerevisiae , brewing , gene , biology , genetics , restriction site , strain (injury) , restriction map , southern blot , yeast , microbiology and biotechnology , plasmid , restriction enzyme , biochemistry , fermentation , anatomy
The gene (POFI) which imparts to certain yeasts the ability to decarboxylate phenolic acids to corresponding phenolic compounds has been analysed by restriction mapping. New restriction sites have been used to examine differences between Pof + and Pof − Saccharomyces cerevisiae strains. Southern Blot analysis of selected yeast strains has demonstrated that the POFI gene sequence is highly conserved between the Pof + strain from which the gene was cloned, two Pof − lager brewing strains and one Pof + Saccharomyces brewery isolate. However, sequence differences have been found between the original Pof + strain, a Pof − laboratory strain and a Pof − ale brewing strain.