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AN INTER‐LABORATORY STUDY OF STARCH DEGRADING ENZYME ASSAYS
Author(s) -
Henry R. J.,
Butler D.
Publication year - 1992
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1992.tb01087.x
Subject(s) - repeatability , amylase , reproducibility , starch , chromatography , enzyme , alpha amylase , chemistry , food science , biochemistry
The reliability of specific colorimetric assays for the main starch degrading enzymes, α‐amylase, β‐amylase and limit dextrinase was investigated by conducting an inter‐laboratory collaborative study. Three malt samples were assayed in duplicate in 14 laboratories. The repeatability (r) within laboratories was good for α‐amylase and β‐amylase assays. Between‐laboratory reproducibility (R) was acceptable (CV R 9.2–14%). Errors for limit dextrinase were higher. Repeatability was good at high enzyme concentrations (CV r = 2%) but increased at low enzyme concentrations. Reproducibility was relatively poor (CV R ‐27–33%).