Premium
COLOUR REACTION SCREENING OF CLONES WITH DIACETYL AND ACETOIN REDUCTASE ACTIVITIES
Author(s) -
Legeay O.,
Ratomahenina R.,
Chabalier C.,
Galzy P.,
Goessens E.
Publication year - 1991
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/j.2050-0416.1991.tb01080.x
Subject(s) - acetoin , diacetyl , chemistry , food science , autoanalyzer , biochemistry , biology , chromatography , fermentation
A genomic library of the chromosomal DNA of a brewing strain ( Saccharomyces carlsbergensis ) has been made in a Escherichia coli HB101 strain as host. A coloured test allowing one to isolate recombined clones possessing diacetyl (DR) and acetoin (AR) reductase activities is described. This test allowed one to detect 3 Escherichia coli clones having both activities (DR and AR) from among 3000 E. coli strains constituting the genomic library of Saccharomyces carlsbergensis in E. coli .